Test Description

ENA Profile-7 comprises of tests for dsDNA, Sm, Scl-70, Chromatin, Centromere, Histone and RNA POL III. An extractable nuclear antigen (ENA) panel detects the presence of one or more auto-antibodies in the blood that react with proteins in the cell nucleus. These proteins are known as “extractable” because they can be removed from cell nuclei using saline. Certain autoimmune disorders are characteristically associated with the presence of one or more anti-ENA antibodies. This association can be used to help diagnose an autoimmune disorder and to distinguish between disorders.

When should this test be ordered?
An ENA panel is ordered when a person has signs and symptoms that could be due to an autoimmune disorder and has a positive ANA test.
High Risk · Outside Reference Range
Greater than reference range:

{Sm, Scl-70, Chromatin, Centromere} : >= 81 (Units)
{Histone, RNA POL III} : >= 2.6 (Units)
{dsDNA} : >= 76 (IU/mL)

Diagnoses of autoimmune diseases are typically based on the characteristic signs and symptoms and on results of autoantibody tests. ENA panel results help to detect and distinguish between different autoimmune disorders.
Borderline Risk · Within Reference Range
Reference Range:

{Sm, Scl-70, Chromatin, Centromere} : 19-80 (Units)
{Histone, RNA POL III} : 1-2.5 (Units)
{dsDNA} : 30-75 (IU/mL)
Low Risk · Within Reference Range
Reference Range:

{Sm, Scl-70, Chromatin, Centromere} : 0-19 (Units)
{Histone, RNA POL III} : 0-0.9 (Units)
{dsDNA} : <= 29 (IU/mL)
Testing Method Used
ELISA.

Please ship samples daily.
Improvements and Prevention
Treatment based on the diagnosis.
Specimen Type
Serum
Fasting Required
No, but may be done with other tests that requires fasting.
Drawing and Processing
  1. Draw from a vein into a SST tubetube1
  2. Invert tube (do not shake) 1-2 times to mix adequately
  3. Let stand upright in tube rack for 30 minutes
  4. Centrifuge for 15 minutes at 3300rpm
  5. Do not leave at room temperature for more than 1 hour
  6. Keep cold in a refrigerator to maintain stability, until ready to ship
Shipping Instructions
  1. Freeze ice packs 24 hours prior to use
  2. Place ice packs inside the Styrofoam insulated kit
  3. Place processed tubes inside biohazard bag, place inside kit
  4. Seal box, print airbill and adhere to box and wait for courier to pick up
Rejection Criteria
QNS; temperature out of range upon receipt; grossly lipemic or hemolyzed; wrong specimen type; sample stability exceeded for this analyte; unlabeled or mislabeled specimen.
References

1. Prince HE, Hogrefe WR (1998). “Evaluation of a line immunoblot assay for detection of antibodies recognizing extractable nuclear antigens”.

The information provided here is intended for educational purposes only and should not be used or substituted as medical advice. You should consult a healthcare practitioner regarding medical diagnosis or treatment.