Test Description

Thyroperoxidase (TPO) is an enzyme involved in thyroid hormone synthesis, catalyzing the oxidation of iodide on tyrosine residues in thyroglobulin for the synthesis of triiodothyronine and thyroxine (tetraiodothyronine). TPO is a membrane-associated hemoglycoprotein expressed only in thyrocytes and is one of the most important thyroid gland antigens.

When should this test be ordered?
Testing may be ordered when an individual has signs and symptoms of a low or high level of thyroid hormones, especially if the cause is suspected to be an autoimmune disease.
High Risk · >= 35 (IU/ml)
Out of Reference Range.
Borderline Risk · None
None determined for this test
Low Risk · <= 34 (IU/mL)
Within Reference Range.
Testing Method Used
Electrochemiluminescence Immunoassay.

Sample is stable for 3 days at 2-8°C.

Please ship samples daily.
Improvements and Prevention
Please consult your physician.
Specimen Type
Serum
Fasting Required
No, but may be done with other tests that requires fasting.
Drawing and Processing
  1. Draw from a vein into a SST tubetube1
  2. Invert tube (do not shake) 1-2 times to mix adequately
  3. Let stand upright in tube rack for 30 minutes
  4. Centrifuge for 15 minutes at 3300rpm
  5. Do not leave at room temperature for more than 1 hour
  6. Keep cold in a refrigerator to maintain stability, until ready to ship
Shipping Instructions
  1. Freeze ice packs 24 hours prior to use
  2. Place ice packs inside the Styrofoam insulated kit
  3. Place processed tubes inside biohazard bag, place inside kit
  4. Seal box, print airbill and adhere to box and wait for courier to pick up
Rejection Criteria
QNS; temperature out of range upon receipt; grossly hemolyzed or lipemic; wrong specimen type; sample stability exceeded for this analyte; unlabeled or mislabeled specimen.
References
1. Gharib H, Tuttle RM, Baskin HJ, et al: Consensus Statement #1, Subclinical thyroid dysfunction: a joint statement on management from the American Association of Clinical Endocrinologists, The American Thyroid Association, and The Endocrine Society. Thyroid 2005;15:24-28

2. Feldt-Rasmussen U: Analytical and clinical performance goals for testing autoantibodies to thyroperoxidase, thyroglobulin, and thyrotropin receptor. Clin Chem 1996;42:160-163

The information provided here is intended for educational purposes only and should not be used or substituted as medical advice. You should consult a healthcare practitioner regarding medical diagnosis or treatment.